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INDONESIA
Jurnal Kimia Terapan Indonesia
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : 08532788     EISSN : 25277669     DOI : -
Core Subject : Science, Engineering,
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Materials Science & Nanotechnology
Arjuna Subject : -
Articles 7 Documents
 1 
Search results for , issue "Vol 13, No 2 (2011)" : 7 Documents clear
PENGARUH KONSENTRASI PENGINDUKSI METANOL SERTA"SUMBER KARBON SORBITOL DAN MONITOL TERHADAP PRODUKSI a-AMILASE Saccharomycopsis fibuligera R64 DALAM Pichia pastoris Shabarni Gaffar; Dani Permana; Diana P Rahmawati; Triana N Meirina; Abu Bakar M.I. Syihab; Safrl Ismayana; Toto Subroto; O Suprijana
Jurnal Kimia Terapan Indonesia Vol 13, No 2 (2011)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5297.927 KB) | DOI: 10.14203/jkti.v13i2.152

Abstract

Pichia pastoris has been widely used as host for heterologous protein expression for commercial purposes. The advantages of P. pastoris as protein expression host is its ability to grow with high cell density and the presence of a gene promoter that canbe induced tightly, named AOXI encoding alcohol oxidase. Improvement of recombinant protein production under control of AOXI promoter in P. pastoris expression system is still a major concern. Optimization of methanol concentration as inducerand addition of carbon sources, is one of the strategies to improve the expression level. This research aims to study the effect of methanol as inducer as well as sorbitol and mannitol as additional carbon source to the expression level of recombinantSaccharomycopsis fibuligera a-amylase (Sfamy) by P. pastoris (Mut). Sorbitol and manuot known as non-repressive carbon source, to increase the growth ofP. past oris, but not inhibit the AOX1 promoter and foreign proteins expression. Sfamy was expressed in P. pastoris GS115 (His-, Mut) with addition ofcarbon source, sorbitol and mannitol saparately to the expression medium. The result showed that, the optimum concentration" of methanol inducer for Sfamy production is 0.75%. The addition of sorbitol or mannitol increased Sfamy production. Concentrations of sorbitol and mannitol 2% with0.75% methanol inducer increase the secretion level of recombinant Sfamy 2.13 times and 1.94 times respectively, compared with no additional carbon source. This result indicated that addition of carbon source can improved recombinant protein production by P. pastoris, and the used of sorbitol as additionalcarbon source is more effective compared to mannitol.Key words: a-amylase. Pichid pastoris, methanol, sorbitol,mannitol.
ISOLASI DAN IDENTIFIKASI SENYAWA FLAVON DARI EKSTRAK ETIL ASETAT BRUCEA JAVANICA Merrill Megawati Megawati; A Darmawan; M Angelina; S Fajriah; M Hanafi; P D Lotulung
Jurnal Kimia Terapan Indonesia Vol 13, No 2 (2011)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4208.568 KB) | DOI: 10.14203/jkti.v13i2.138

Abstract

Buah Makasar (Brucea javanica Merrill)is a wild ornamental plant and one of the traditional medicine plants. Isolation andpurification of ethyl acetate extract of B.javanica using silica gel column chromatography with ethyl acetatem-hexane as a mobile phase gradually obtained a pure compound as yellow crystals. It was identified by using infrared spectrophotometer (IR), LC-MS (liquid chromatography mass spectrometer) and ID- and 2D-NMR (nuclear magnetic resonance), compound a identified as 6-hydroxy-2-(3,4,5-trihydroxyphenyl}-4H-chromen-4-one, a flavones compound with molecular formula Cl5HI006 and molecular weight of 287.18 m/z.Keywords: Brucea javanica Merrill, buah makasar, flavones,6-hydroxy-2-(3,4,5-trihydroxyphenyl)-4H-chromen-4-one
EKSTRAK TERSTANDAR SECARA KIMIA DAUN Brucea javanica Merrill Marissa Angelina; Abdul Mun'im; M Hanafi
Jurnal Kimia Terapan Indonesia Vol 13, No 2 (2011)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4677.355 KB) | DOI: 10.14203/jkti.v13i2.143

Abstract

The preparation of Brucea leaves have been done for specific parameters and non specific parameter laboratory worb refers to TheNational Agency of Drug and Food Control Regulation for extract quality control. The result for specific extract parameters  were the rendement of extraction is 28%, the values of water- extractive and ethanol-extractive are 11.49% and 9.41%,  respectively. The result of determination of the non specific  parameters of the extract are; loss on drying is 18.95%, the  water content is 15.06%, the total ashes is 16.12%. and the  level of ashes not dissolved in acid is 11.14%. The analysis of chemical compound shows that the extract containedflavonoid,  tannin, and glycoside. For the quantitative control has been measured the level of totalflavonoid wherethe result is 9.901%Keywords : Preparation, Brucea; Specific parameter, non specificparameter, extract
PENGARUH SUMBER KARBON TERHADAP PRODUKSI ENZIM INULINFRUKTOTRANSFERASE DARI Nonomuraea sp Yetti Mulyati I; Sri Pudjiraharti; Een Sri Endah; Tanaka tanaka; Teruo Sone; Asano K
Jurnal Kimia Terapan Indonesia Vol 13, No 2 (2011)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4214.295 KB) | DOI: 10.14203/jkti.v13i2.148

Abstract

The aims of this research is to produce inulinfructotransferase enzyme with potentially benefit of bacteria Actinomycetes which was isolated from Indonesia soils to produce Difructoanhydride-Ill (DFA III), a compound has physiology activities to enhance calsium absorption of rats. cows and human colons so that the materials can be applied as antiosteoporosis. The processing of inulinfructotransferase enzyme from  Nonomuraea sp by usingshakeflask level and study the influence of carbohydrate source were conducted. This enzyme has  optimum temperature at 65rtC,higher than optimum temperature which has found before and the activities was stable  at 70dc for 20 minutes. So that the enzyme has  potentially applicable for industry scale. The results indicated that optimation condition for processing inulinfructotransferase enzyme of Nonomuraea sp : temperature sore.  incubation time 48 hours.carbon source of inulin 20 g/L, nitrogen source 5g/L, noyeast extract added, the enzyme activities was 7,3 Unit/ml;  However,when medium contains yeast extract 0,2 gIL,the  inulinfructotransferase enzyme activity was 14,6 Unit/mL.Keywords: Inulinfructotransferase enzyme, Nonomuraeasp.inulin. yeast extract, DFAII
ANTICANCER ACTIVITY OF ETHANOLIC EXTRACT OF Selaginella plana HIERON. ON T47D CELL LINE IN VITRO Chandra Risdian; Sri Handayani; Zalinar Udin; Rina Andriyani; Marissa Angelina
Jurnal Kimia Terapan Indonesia Vol 13, No 2 (2011)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3718.065 KB) | DOI: 10.14203/jkti.v13i2.153

Abstract

Selaginella sp belongs to the Selaginellaceae family. It has been used in China and Indonesia as a traditional medicine. It hasseveral medicinal properties including antibacterial, anticardiovascular, and anticancer agent. The aim of the present study was to access the anticancer property of the ethanolic  extracts of Selaginella plana Hieron. on T47D breast cancer cell line. The proliferation of T47D cell line was detected by SRB (Sulforhodamine B) assay which was measured at a wavelength of 515 nM. The result showed that the IC50 of the ethanolic extract was determined at 7.03 pg/mL. This significant activity was assumed due to its high total flavonoid content. The totalflavonoid content of the ethanolic extract was 23.04%. Flow cytometry analysis indicated that the extract may undergo the cell death via apoptosis pathway. In conclusion, the ethanolic extract of Selaginella plana Hieron: has considerable activity in inhibiting T47D cell line proliferation.Key words: Selaginella plana Hieron., SulforhodamineB, T47Dcells, anticancer flow cytometry
PENGARUH ELEKTROLISIS TERHADAP RENDEMEN MINYAK JARAK YANG DIHASILKAN PADA PROSES PEMBUATAN BIODIESEL Herlian Eriska Putra; Agusta Samodra Putra
Jurnal Kimia Terapan Indonesia Vol 13, No 2 (2011)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jkti.v13i2.154

Abstract

The use of jatropha oil as a source of raw material for making biodiesel is needed in order to decrease the production cost of biodiesel, since jatropha oil is categorized as non edible fat. In this research, the biodiesel production process by using an electrochemical reactor has been done. At the beginning of treatment, carried out the hydrolysis of oil by using HrS04 with acid and oil volume ratio is 1: 20. At follow-up treatment, carried out the decarboxylation via electrolysis. This process is done by varying the voltage of 0 V, 10 V,20 V,30 V,the variation of the concentration of 0.5 M acetic acid. 1 M, 1.5 M, as well as the distance between the electrodes in the reactor 5em and 10 em. From the experiments shown that electrolysis with voltage  parameters, acidic electrolyte solution. and electrode spacing affects the yield of decarboxylation process. Electrolysiswith 1.5 M acetic acid concentration and voltage of 30 volts per cent obtained the highest biodiesel yield of compound that is 70.432% in the reactor with inter-electrode distance of 5cm. While in the reactor with inter-electrode distance of 10 cm. thehighest yield was 53.372% by reacting the oil with 1.5 M acetic acid and voltage 30 Volt.Keywords : voltage, decarboxylation, electrolysis, acidconcentration, electrode distance, biodiesel.
STUDI AWAL PADA PREPARASI KATALIS BERBASIS LEMPUNG BERPILAR UNTUK REAKSI ETANOL MENJADI GASOLINE (ETG) Nino Rinaldi; Adid Adep Dwiatmoko
Jurnal Kimia Terapan Indonesia Vol 13, No 2 (2011)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5789.37 KB) | DOI: 10.14203/jkti.v13i2.155

Abstract

Research on catalytic reaction of ethanol to gasoline (ETG) was investigated in this study. The catalyst with a high acidity is required for the ETG reaction. Therefore, this study focused on the catalyst preparation of ETG reaction by using natural clays which prepared by the pillarization techniquewithseveral metal oxides, such as:AI, Zr, Fe and Cr as the pillared. The prepared of pillared clay were characterized by x-ray diffraction, specific surface area and pore distribution (BET) measurements and  catalytic activity testing on the ETG reaction. It is observed that after the pillarization; the surface area and basal spacing (d001) of the natural clays were significantly increased. thus the   ptllarization process was successfully prepared on natural clay. However,the acidity of theprepared ofpillared clay is still  lower,probably due to the cations between layers of the natural clay were not homogeneous, thus although the pillarization process was happen but still not completely. Moreover, IR measurement showed only the acid type of Lewis is increased after the pillarization. Thistype is not favorable for the ETG reaction, and thus only the lower hydrocarbon products (<Cl2) were observed after the ETG reaction, even aromatics and cyclic hydrocarboncompounds wereobtainedKeyword : preparation, pillared clay, catalyst, ETG reaction.

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